Bone Marrow Concentrate (BMC) Products

The ART BMC Plus:

The ART BMC Plus features an integrated filter system for the ultrafiltration of proteins from platelet poor plasma. By injecting the plasma from one syringe on Port “B “to another syringe on Port “C” the cells are forced through the hundreds of hollow, nanoporous fibers, and water and salt ions are pushed out while proteins and cells are further concentrated. The fluid may be concentrated by passing through the filter until the desired volume is obtained. This ultrafiltration enriches the concentration of most proteins like Alpha-2 Macroglobulin (A2M), Interleukin-1 receptor antagonist protein (IRAP), VEGF, PDG-F, TGF-Beta, FGF and Fibrinogen1, 2, 3.

A2M is a powerful natural plasma protease inhibitor, cytokine carrier, and ligand for cell-signaling receptors, which exists in two well-characterized conformations and is synthesized primarily in the liver but dispersed through the blood plasma in bone marrow and blood4 . A2M stops the progression of Osteoarthritis (OA) by preventing degenerative cartilage breakdown and assisting with cartilage repair 4. In its plasma-soluble tetramer form, it encapsulates matrix metallo-proteinases (MMP’s) and other destructive enzymes common to arthritic tissue and slows or halts the breakdown of articular cartilage surfaces and other forms of the Extracellular Matrix (ECM)5 . A2M also binds up pro-inflammatory molecules TNF-a, TGF-b, and IL-1b 4.

ART BMC Family Features:

• Nearly closed systems offers multiple connection ports

• Thumb-wheel allows precise recovery of the targeted cell layer

• Large aperture collection window

• Manual collection accommodates full spectrum of hematocrit levels

• Adjustable flow valve diverts fluid without additional sterile breaks

• Adjustable flow valve simplifies separation/combining of cells and proteins

• ART PRP TM Plus concentrates platelet poor plasma (PPP) using nanoporous fibers

• ART PRP TM Plus reduces 60 mL whole blood to 25-30 mL of PPP and nano-filtration further concentrates to 3-5 mL concentrated PPP

ART BMC Family Benefits:

• Minimizes sterile breaks where contamination occurs

• Easy separation of fluid fractions (e.g. PRP, PPP); Processes anywhere from 50 cc up to 60 cc of whole blood

• Eliminates turbulence reducing cell velocity that can lyse cells• Increases cell recovery rates; other fully automated designs compromise recovery rate

• Keeps Buffy Coat in the collection zone yielding 3 cc to 5 cc on average of PRP (Buffy Coat) from a typical 60 cc volume of whole blood

• Manual manipulation is the critical feature that produces optimal recovery and a customized final product

• Provides for the highest concentration so that more cells can be delivered in the smaller volume

‍

The ART BMC System: processes autologous bone marrow aspirate into bone marrow concentrate effectively and efficiently at point-of-care. The system recovers the greatest percentage of nucleated and progenitor cells by allowing selection of the cell concentrate from user-defined portions of the centrifuged stack. This system is a miniaturized laboratory in a single-use sterile device. The back chamber may be used to store the platelet poor plasma without breaking sterility.

Focused on the safe handling of cells, the ART BMC has multiple ports to accommodate the fewest sterile breaks possible, so that the valuable recovered cells are exposed to the minimum circulating air contaminants. The built-in back chamber allows for the convenience of adding platelet poor plasma (PPP) back to the bone marrow concentrate (BMC) should more volume be desired for injection; all without additional sterile breaks.